Grow Supplies

 Instructions

Medicinal Herbs Home Test Kit

Novel chemical compound assay

Area Prep:

1.) Remove all items from the area not needed for your testing.

2.) Collect materials not included in the kit needed for testing.

a) Thermometer

b) A scale accurate to .1g

c) Timer, or some way to keep track of elapsed time

d) Some way to capture an image of the T.L.C. plates after being sprayed with the T.L.C. dye.

i.  Flat scanner

ii. Digital camera

Now you are ready to start some testing!!

WEAR GLOVES!!

Sample Prep:

1.) Make sure samples are DRY and crumbly. The greater the moisture content the less accurate the T.L.C. method is.

2.) Weigh dry samples at a volume you can reproduce accurately. The volume should be less than 500mg for this test kit.

3.) Place samples in the 1.5mL tubes and add 1mL of the Extraction Solution. Crush and vigorously shake the samples. Let sit for ~5min.

T.L.C. Plate Prep:

1.) Only handle T.L.C. plates with gloved hands.

2.) Make marks on the plate with a sharp pencil at least ½” above the bottom of the plate. You do not want your samples in the Developing Solution when you place the T.L.C plates into the Developing Chamber.

3.) Load your desired volume of samples no less than 1 microliter no greater than 3 microliters. Be consistent with volumes loaded on the sample plate.

T.L.C. Plate Developing Prep:

1.) Place the contents of the developing solution bottle into the jar/developing chamber.

2.) Place the chamber on a level surface, and in an area where it will not be disturbed for ~15-20 minutes.

2A for heated assay only) Heat plates in a 300°F preheated oven for ~3.5 minutes. Remove from heat and let cool.

3.) Place T.L.C. plate into the chamber, carefully. Place the lid on the developing chamber, carefully. Let the plate run for ~15-20 minutes, or until the developing solution is ¼” from the top of the T.L.C. plate.

4.) Remove from the chamber and let air dry before using the T.L.C dye.

T.L.C. Dye Prep:

This is a DYE and WILL stain!!

1.) As the T.L.C. plates are running in the developing chamber, prepare your T.L.C. dye.

2.) Add 1mL water to the T.L.C. dye 1.5mL tube. Shake vigorously. Let set.

3.) Add 4mL of water to the T.L.C. sprayer.

4.) Transfer the 1mL T.L.C. dye concentrate to the T.L.C. sprayer. Shake vigorously. Let set.

5.) Use the Hydroponic Herb’s™ box as a spray chamber.

6.) One 5mL sprayer will dye 2 plates.

7.) Spray plates as you practiced.

8.) Let air dry.

Okay, you are almost done.

Capturing an Image:

By this point you have chosen a way to image your plates so you can save and analyze the data at your leisure. Have the plate imaged at least 15 min after dying. If not, results are unreliable. E-mail an image of your slide to hydroponicherbsupplies@gmail.com for free qualitative analysis. Your results will be e-mailed back to you promptly. If you are interested in qualitative results please click contact us.

Medicinal Herbs Mail-In Test Kit™

 Novel compound analysis assay. 

Area Prep

1.) Remove all items from the area not needed for your testing.

2.) Make sure you have a scale capable of measuring down to .1 grams

Now you are ready to start some testing!!

WEAR GLOVES!!

Sample Prep:

1.) Make sure samples are DRY and crumbly. The greater the moisture content the less accurate the T.L.C. method is.

2.) Weigh dry samples at a volume you can reproduce accurately. The volume should be less than .5 grams for this test kit. This kit will only fit two samples per plate.

3.) Place samples in the 1.5mL tubes and add 1mL of the Extraction Solution. Crush and vigorously shake the samples. Let sit for ~5min.

T.L.C. Plate Prep:

1.) Only handle T.L.C. plates with gloved hands.

2.) Load your desired volume of samples no less than 1 microliter no greater than 3 microliters into the prepared marks on the plate. Be consistent with volumes loaded on the sample plate.

3.) Be sure to mark on the back with a permanent marker a number 1 on one plate and a number 2 on the other. Make a key so as to remember the order of the samples submitted. A copy of the key should be submitted. Be sure to keep track of the volume weighed, and the volume loaded onto the slide.

3.) Place your slide back into the plastic shipping container making sure the plate is protected from jostling by using the packing paper we have included. “Hydroponic Herb’s™ is not responsible for improperly packed slides and no refunds will be issued.”

4.) Place the plastic shipping container into the return envelope. Mail back to:

Hydroponic Herb’s™

817 West Koch Street

Bozeman Montana, 59715.

Your qualitative results will be e-mailed to you. If you are interested in the percentage by volume of novel compounds within your samples please click contact us.

Wholesale

Hydroponic Herb’s™ Medicinal Herbs Home Test Kit™ Wholesale Pricing:

Hydroponic Herb’s™ Medicinal Herbs Home Test Kit™ Wholesale Pricing:

Price per unit USD Quantity Guarantee

$100

1-4 none

$50.00

5-10 90 day buy back on unsold units

$40.00

11-25 90 day buy back on unsold units

$30

26-50 90 day buy back on unsold units

$25

51+ 90 day buy back on unsold units
Shipping USD
$3.50 per unit within the US $50.00 Outside the US

Send your order as a reply to HydroponicHerbSupplies@gmail.com. We will reply within 24 hours with a secure PayPal link to finalize your wholesale transaction. We  have consignment options as well, so be sure to call and ask for more information.

We will be glad to answer any questions you may have. Thanks again!

Web: HydroponicHerbSupplies@gmail.com
Twitter: @HydroponicHerbs

Phone: 406-599-4379

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